Equipment

The Institute of Immunology provides a range of scientific instruments that are available for use upon request.

If you are interested, please contact us at:

immunologie{bei}med.uni-rostock.de.

 

Analytics:

Microplate Reader Infinite® M Plex 200 PRO

The Infinite M Plex is a fully equipped multi-mode plate reader with monochromator-based optics that offers free wavelength selection and scanning capabilities. It is a ready-to-use workhorse for all standard absorption, fluorescence, and luminescence assays.

Applications:

  • Absorption- and fluorescence-based protein quantification
  • Absorption-, fluorescence-, and luminescence-based ELISAs
  • 600-nm growth curves (bacteria, yeast)
  • ATP quantification
  • Fluorescence- and luminescence-based reporter assays
  • Fluorescence- and luminescence-based Ca²⁺ release assays
  • Dual-luciferase reporter assays
  • Cell-based assays (e.g., viability, cytotoxicity)
  • Enzyme kinetics
  • Characterization of active compounds (absorption and fluorescence scans)

Configuration:

  • Absorption: endpoint, kinetics, spectral measurement (Monochromator-based only)
  • Monochromator: Quad4 monochromators, 230 nm – 1,000 nm
  • Fluorescence: endpoint, kinetics, spectral scan (monochromator-based only), TRF, FRET
  • Monochromator: Quad4 monochromators, Ex 230 nm – 850 nm, Em 280 nm – 850 nm
  • Luminescence
  • Standard: glow, flash (with injectors), two-color measurement, BRET2™, NanoBRET™, OD2 attenuation
  • Injector: 1 or 2 pumps, 1,000 μl pumps
  • Incubation Options
  • Shaking
  • Heating: RT +5°C to 42°C
  • Software
  • i-Control™: Reader Control Software
plate washer
SeqStudio Genetic Analyzer

SeqStudio Genetic Analyzer

The SeqStudio Genetic Analyzer is a 4-capillary electrophoresis system with single-base resolution (Sanger sequencer). The four capillaries, the universal polymer, and the anode buffer are contained in a replaceable cartridge that allows for the analysis of 1,000 samples. The system can measure the emission of 6 fluorescent dyes and is currently calibrated for 6FAM™ dye, VIC® dye, NED™ dye, PET® dye, and LIZ® dye.

  • Sanger sequencing with BigDye Terminator v1.1, ~v3.1, BigDye Direct
  • Fragment analysis
  • DNA methylation analysis (Bisulfite sequencing)
  • MSI (microsatellite instability) analysis
  • Multiplex SNP analysis using the Snap Shot method
  • MLPA analyses (multiplex ligation-dependent probe amplification)

Use of the system may require the purchase of a replacement cartridge and other necessary consumables, as well as an introduction by the person responsible for the equipment.

Sample preparation:

Agilent BioTek 50 TS (96-well plate) Washer

The Agilent BioTek 50 TS Washer delivers precise and reproducible results for the automated washing of 96-well microplates.

Applications

  • ELISA
  • Biomagnetic particle separation assays
  • Cell-based assays

 

Technical Details

  • Microplate types: 96-well plates and microplate strips
  • Shaking: Programmable in minutes and seconds up to 30 minutes; 5 intensity levels from 15 to 19 Hz
  • Soaking: Programmable in minutes and seconds up to 30 minutes
  • Separation methods: Biomagnetic separation
  • Manifold types: 8-well manifold
  • Volume range: 25–3,000 μl per well
  • Wash speed: < 130 s for 12 strips (3 cycles, 300 μl/well, without soaking)
  • Dosing accuracy: 3.0% CV when measured over six doses of 300 μl/well of deionized water with 0.1% Tween 20.
  • Residual volume: ≤ 2.0 μl/well after a 3-cycle wash run, 300 μl/well dispensed
Agilent BioTek 50
FastPrep 24 Homogenizer

FastPrep 24 Homogenizer

The FastPrep 24 Homogenizer allows for the simultaneous homogenization of up to 24 soft tissue samples.

  • Soft tissue, e.g., muscle, kidney, spleen, brain, etc. (not for cartilage, bone, frozen samples, etc.) in disposable 2-ml screw-cap vials with ceramic beads (lysis matrix)
  • In combination with a lysis buffer, the use of fresh tissue, or prior preservation in RNAlater (RNA)
  • Extraction of RNA, DNA, and proteins
  • Special consumables are required (MP Lysing Matrix Tubes)

Covaris M220, programmable Sonifier

Covaris M220, programmierbarer Sonifier

The Covaris M220 is a programmable ultrasonic device designed for use with isothermal AFA (Adaptive Focused Acoustics) technology.

  • Shearing of nucleic acids for NGS fragment library construction
  • Shearing of DNA and chromatin for ChIP-seq
  • Extraction of DNA and RNA from FFPE material (truXTRACR FFPE Extraction Kits)
  • Sample extraction (prokaryotes, eukaryotes)
  • Special consumables have to be purchased 

High-Performance Centrifuges

Beckman Coulter Optima™ Max

Rotors:

  • MLA-80 (fixed-angle); 8 x 8 ml capacity; max. 444,000 g at 80,000 rpm
  • MLS-50 (swing-out); 4 x 5 ml capacity; max. 268,000 g at 50,000 rpm
  • MLS-130 (fixed-angle); 10 x 2 ml volume capacity; max. 1,019,000 g at 130,000 rpm
Ultrazentrifuge OptimaTM Max von Beckman Coulte

Beckman Coulter Avanti™ J-25 Benchtop refrigerated centrifuge

Rotors:

  • JA-25.50 (fixed-angle); 8 x 50 ml capacity; max. 75,600 g at 25,000 rpm
  • JLA-16.250 (fixed-angle); 6 x 250 ml capacity; max. 38,400 g at 16,000 rpm
Beckman Coulter AvantiTM J-25 Benchtop refrigerated centrifuge

Quantification and Integrity Control:

NanoDrop One Spektralphotometer - ThermoFisher

NanoDrop™ One Spektral Photometer - ThermoFisher

The NanoDrop One is a UV/Vis microvolume (1 to 2 μl) spectrophotometer for measuring absorbance in the range of 190 to 850 nm.

  • Quantification of nucleic acid- and protein samples is possible in a linear range of up to 27,500 ng for DNA/RNA and 400 mg/ml IgG without dilution
  • “Acclaro Sample Intelligence Technology” enables the differentiation of DNA and RNA as well as automatic correction for potential contaminants
  • Determination of peptides (205 nm), proteins (280 nm), DNA and RNA (260 nm), dye analysis (490 nm), gold nanoparticles (520 nm), colorimetric protein assays (BCA, 562 nm; Bradford, 595 nm; Lowry, 650 nm; Pierce 660, 660 nm), and optical density measurement (600 nm)
Qubit Fluorometer

Qubit™ Fluorometer

The Qubit™ Fluorometer is a fluorescence photometer that enables the quantification of RNA, DNA, and proteins using a two-point regression and the appropriate dye kits.

Kits/assays used:

  • Qubit dsDNA HS Kit (0.2 to 100 ng)
  • Qubit dsDNA BR Kit (2 to 1000 ng)
  • Qubit RNA HS Kit (5 to 100 ng)
  • Qubit RNA BR Kit (20 to 1000 ng)
  • Qubit Protein Assay (0.25 to 5 μl)
  • Qubit Protein BR Assays (1 to 400 μl)
Agilent TapeStation 4150

Agilent TapeStation 4150

The TapeStation 4150 is an automated electrophoresis system for the quality control of nucleic acids, succeeding the “lab-on-a-chip” technology (Bioanalyzer 2100)

  • Integrity assessment of RNA and DNA samples
  • RIN values (RNA Integrity Number); DIN values (DNA Integrity Number); DV200 value
  • 16 separation lanes can be processed consecutively using barcodes


Kits/assays used:

  • RNA Assay (25 to 500 ng, sensitivity 5 ng)
  • High Sensitivity RNA Assay (500 to 10,000 pg, sensitivity 100 pg)
  • D1000 Assay (0.1 to 50 ng)
  • High Sensitivity D1000 Assays (sensitivity 5 pg)
  • D5000 Assay (0.1 to 50 ng)
  • High Sensitivity D5000 Assay (sensitivity 5 pg)
  • Cell-free DNA Assay (50 to 800 bp)
  • Genomic DNA Assay (200 to > 60,000 bp)
Agilent Bioanalyzer 2100

Agilent Bioanalyzer 2100

The Agilent Bioanalyzer is an automated capillary electrophoresis system (lab-on-a-chip) for the analysis of nucleic acids (DNA, RNA) and proteins.

  • Integrity assessment of RNA and DNA samples
  • RIN values (RNA Integrity Number); DIN values (DNA Integrity Number)


Kits/assays used:

  • Agilent RNA 6000 Nano Kit (25 to 500 ng)
  • Agilent RNA 6000 Pico Kit (50 to 5000 pg)
  • Agilent Small RNA Kit (6 to 150 nt)
  • Agilent DNA 1000 Kit
  • Agilent DNA 7500 Kit
  • Agilent DNA 12000 Kit
  • High Sensitivity DNA Kit (5 to 100 pg)
  • Agilent Protein 80 Kit (< 80 kDa)
  • Agilent Protein 230 Kit (< 230 kDa)
  • High Sensitivity Protein 250 Kit (< 250 kDa; 0.3 to 3000 ng)